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KEY RESOURCES TABLE

Journal: Structure (London, England : 1993)

Article Title: Conservation of Dynamics Associated with Biological Function in an Enzyme Superfamily

doi: 10.1016/j.str.2018.01.015

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biological Samples pJExpress414 vectors Atum (Newark, CA) https://www.atum.bio/products/expression-vectors/bacterial?hl=pJExpress%20414#pg41 pET-22b(+) DNA EMD Millipore (Billerica, MA) 69744 Chemicals, Peptides, and Recombinant Proteins 15 N-ammonium chloride Cambridge Isotope Laboratory (Andover, MA) NLM-467-PK Ampicillin, sodium salt BioBasic (Markham, ON, Canada) AB0028 L-arginine monohydrochloride BioBasic (Markham, ON, Canada) AB0206 Deuterium oxide Sigma (St. Louis, MO) 151882 Dextrose Sigma (St. Louis, MO) D9434 Ethylenediaminetetraacetic acid 0.5 M sterile solution BioBasic (Markham, ON, Canada) SD8135 13 C-glucose Cambridge Isotope Laboratory (Andover, MA) CLM-420-PK Glutathione, oxydized BioBasic (Markham, ON, Canada) GB0228 Glutathione, reduced BioBasic (Markham, ON, Canada) GB0229 Guanidine hydrochloride BioBasic (Markham, ON, Canada) GB0242 Isopropyl ß-D-thiogalactopyranoside BioBasic (Markham, ON, Canada) IB0168 MEM vitamin solution Sigma (St. Louis, MO) M6895 2-( N -Morpholino) ethanesulfonic acid sodium salt Sigma (St. Louis, MO) M3885 Magnesium sulfate Sigma (St. Louis, MO) M7506 Sodium acetate Sigma (St. Louis, MO) S2889 Sodium chloride Sigma (St. Louis, MO) S7653 Thiamine hydrochloride (B 1 ) Sigma (St. Louis, MO) 47858 Tris base Sigma (St. Louis, MO) 648311 Experimental Models: Organisms/Strains E. coli BL21 (DE3) New England Biolabs (Ipswich, MA) C2527I Software and Algorithms NMRPipe ( Delaglio et al., 1995 ) https://www.ibbr.umd.edu/nmrpipe CcpNmr ( Vranken et al., 2005 ) http://www.ccpn.ac.uk/v2-software/software/analysis Sparky ( Goddard and Keneller, 2008 ). https://www.cgl.ucsf.edu/home/sparky/ ClustalΩ ( Sievers et al., 2011 ) www.ebi.ac.uk/Tools/msa/clustalo/ Figtree v1.4.2 N/A http://tree.bio.ed.ac.uk/software/figtree/ MODELLER v9.16 ( Webb and Sali, 2014 ) https://salilab.org/modeller RaxML v8.0.26 ( Stamatakis, 2014 ) https://sco.hits.org/exelixis/software.html AMBER v14 ( Case et al., 2014 ) http://ambermd.org/ GraphPad Prism 6 N/A https://www.graphpad.com/scientific-software/prism/ Others HiTrap Q HP anion exchange columns GE Healthcare (Piscataway, NJ) 17115401 HiTrap SP HP cation exchange columns GE Healthcare (Piscataway, NJ) 17115201 Cellulose ultrafiltration membrane EMD Millipore (Billerica, MA) PLBC07610 Open in a separate window KEY RESOURCES TABLE

Techniques: Recombinant, Sterility, Software, Membrane